BioMould Silage Preservative - Feedar Group
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silage ,protein,Preservative ,lactobacilli ,BioMould

BioMould Silage Preservative

BioMould®

Silage Preservative


Proper Fermentation Stimulant

Ensiling is the preservation of forage based on lactic acid fermentation under anaerobic conditions. The lactic acid bacteria ferment water-soluble carbohydrates in the crop to lactic acid, and to a lesser extent to acetic acid. The production of these acids reduces the pH of the ensiled forage which inhibits spoilage microorganisms’ growth and proliferation (table 1).

One of the factors of a proper management for providing good silage is supplementation of that with some additive. Organic acids are used directly (all over or just on the surface) in the silage, in order to protect it by reduction in PH. Propionic acid and formic acid are mostly used for products containing more than 70% moisture and low in glucose such as maize which is high in moisture.

Table 1: Fermentation process in corn silage

PhasesAerobicAnaerobicStable
Days1234-78-21After day 21
EventsCellular respiration and production of CO2, heat and waterInitiation of fermentation, acetic acid production and temperature reductionInitiation of lactic acid production and continuous of acetic acid productioncontinuous of lactic acid production and temperature reductionContinuous of lactic acid and PH reduction of silage and then PH stabilizing.Termination in bacterial fermentation until silage expose to oxygen
Temperature70OF95OF80-85 OFEnvironmental temperature
PH65PH reduces to 4 and stabilize in this level

Formic acid, (43% in BioMould®) as a potential bactericide, improves protein preservation of silage by an immediate acidification which results in inactivating of protein-degrading bacteria. Propionic acid, (16% in BioMould®), as a fungicide, improves dry matter recovery and feedout stability of silage by inhibiting yeast and mold growth as yeast cannot assimilate propionic acid.

 


Benefits of adding BioMould® to the silage

  • BioMould® contains High concentration of formic acid and propionic acid (57%)
  • Reduction in PH by applying BioMould® in silage results in Lactic acid bacteria propagation which subsequently induces resistance against Pathogenic molds, Butyric acid bacteria and Clostridia Spp.
  • The carrier material releases the acids mainly as gas, so that the acid vapors are equally distributed throughout the silage to be reserved.
  • It Increases nutritional value of silage by preserving its crude protein content.
  • BioMould® induces quick lactic acid bacteria fermentation which results in protecting nutrients of silage.
  • Consequently BioMould® boosts the durability of silage.


Surface and edge treatment of silage

An effective additive may help make good silage better, but it will not make poor silage good. The options to create better silage are manifold. One efficient method is surface treatment of silage.

The treatment of silo surface does not replace complete treatment at ensiling, but, if used properly, it is an efficient protection against losses at the especially endangered parts of the silo. In one study spreading 0.5 kg/m2 on corn silo surface was tested.

Spreading BioMould® on top of the ready to ensile stock is possible, if the silage is sealed air tightly immediately afterwards. Due to the especial product formulation the acids diffuse slowly from the Vermiculite lattice (from the carrier) and concentrates below the silage-film. As the acidic vapors cannot escape, the acids concentrate in the upper layer of the silage and so provide efficient protection. Acid formic in BioMould® caused reduction in aerobic bacteria and clostridia growth and simultaneous increase in Lactobacilli population (table 2). Reduction in yeasts and molds growth demonstrates that volatile acids like propionic acid are better inhibitors for these organisms than lactic acid (table 3).

Table 2- Comparing Means (Test–Duncan) of bacterial cultures in samples treated with and without BioMould® (P<0.05)
 Lactobacilli

(MRS) (CFU/g)

Aerobic bacteria

(NA) (CFU/g)

Clostridia

(Blood Agar) (CFU/g)

Dilution of Microbial Suspension

Treatment

104105106104105106104105106
Surface Control (without BioMould®)(260.25 × 105)b(270.00 × 104)b(255.25 × 103)b(325.75 × 104)a(307.75 × 103)aHemolysis (β)Hemolysis (β)Hemolysis (β)
Depth Control

(without BioMould®)

(334.50 × 105)a(322.00 × 104)a(324.00 × 103)a(222.50 × 105)a(213.25 × 104)b(214.50 × 103)bHemolysis (β)Hemolysis (β)Hemolysis (β)
Surface Treatment (with BioMould®)(167.50 × 105)d(166.50 × 104)d(168.75 × 103)d(81.50 × 105)b(83.50 × 104)c(84.00 × 103)cHemolysis (γ)Hemolysis (γ)Hemolysis (γ)
Depth Treatment

(with BioMould®)

(206.50 × 105)c(204.50 × 104)c(201.50 × 103)c(14.75 × 105)c(15.00 × 104)d(11.50 × 103)dHemolysis (γ)Hemolysis (γ)Hemolysis (γ)

Table 3- Comparing Means (Test–Duncan) of yeast and mold cultures in samples treated with and without BioMould® (P<0.05)
 Yeast (CFU/g)Mould (CFU/g)
Dilution of Microbial Suspension

Treatment

101102103101102103
Surface Control (without BioMould®)(148.70 × 103)a(133.75 × 102)a(121.50 × 101)a(69.75 × 103)a(61.00 × 102)a(57.25 × 101)a
Depth Control

(without BioMould®)

(110.00 × 103)b(108.50 × 102)b(100.50 × 101)a(30.50 × 103)c(26.00 × 102)c(24.00 × 101)c
Surface Treatment (with BioMould®)(95.50 × 103)b(90.50 × 102)b(86.25 × 101)ab(42.50 × 103)b(41.75 × 102)b(40.50 × 101)b
Depth Treatment

(with BioMould®)

(76.25 × 103)c(69.75 × 102)c(65.75 × 101)b(22.00 × 103)d(20.25 × 102)d(16.00 × 101)d

In this study, applying BioMould® in the surface of silage lead to reduction in growth of aerobic bacteria and clostridia Spp. because of formic acid effects: reduction in PH and increase in Lactobacilli population growth. The ratio of population of lactobacilli SPP. to population of aerobic species of silage is the most significant parameter for determining the quality of silage surface (table 4).

Table 4- Effects of Biomould® on Lactobacilli to aerobic bacteria ratio
 Lactobacilli to aerobic bacteria ratio
Dilution of microbial suspension104105106
Surface2.4062.426
Depth9.1469.02911.600

Table 5- Fermentation parameters of corn silage subsequent to adding Biomould®
Treatments/effects mg/dLg/100gFW*
PHN-NH3LactateAcetatePropionateButyrateIsovalerateValerate
Control4.095a2.077a1.921a2.090a0.167a0.054a0.039a0.041a
BioMould®3.924a1.770a2.055a2.624a0.280a0.023b0.014b0.079a
Significance level0.3260.5800.7730.0650.1940.01030.0460.459
standard error of the mean0.08350.10680.19310.1310.04270.00520.00560.0123
Surface4.116a1.871a2.048a2.248a0.229a0.029a0.028a0.040a
Depth3.903a1.770a2.317a2.466a0.244a0.048b0.025b0.080a
Significance level0.2350.7180.3210.4210.8690.0930.8280.430
standard error of the mean0.08360.13690.24210.13120.04460.00510.00560.0245

FW: Fresh Weight

Table 6- Chemical analysis of corn silage subsequent to adding Biomould®
Treatments/

effects

OC%
TemperatureDMOMCPAshEENDFADFNFC
Control18.771a21.181b92.814a9.130a7.186a2.941a50.716a23.828a30.186a
BioMould®17.238a25.256a92.533a9.314a7.468a2.845b49.253a22.738a31.121a
Significance level0.3660.00060.4220.5160.4210.4760.4590.1390.763
standard error of the mean0.04180.42650.1690.14580.1680.06520.80720.3311.0525

Adding BioMould® to silage resulted in lactate production due to increase in lactobacilli population. Domination of these species in the silage is directly related to the acidic environment provided by BioMould® (table 5). Wasting energy and protein in silage by Lactobacilli Spp. is lesser than other bacterial species. Simultaneously, BioMould® decreases PH of silage immediately by reducing buffer capacity. This is followed by inhibiting growth yeasts, molds and pathogenic bacteria like clostridia Spp. These microorganisms are butyrate producers and the main protein-degrading factors in the silage.


BioMould®-treated silage

Ammonia reduction in BioMould®-treated silage is another indicator that demonstrates success in preserving the protein content of silage. Final products of fermentation not only indicate the quality of silage, but also demonstrate the kind of fermented sugars and dominated microorganisms in the silage. Accordingly, amount of lactic acid in the silage is a criterion for its soluble carbohydrate content that can help proper ensilaging.

In this study, adding BioMould® to the silage resulted in significant increase in dry matter content (table 6). It should be noted that propionic acid is the factor for preserving dry matter content and aerobic stability of silage by preventing from yeast and mold growth (propionic acid is a fungicide). Increase in none-fibrous carbohydrates and crude protein is a criterion for increase in dry matter of silage. Formic acid preserved protein content of silage by immediate acidifying and subsequently prevention of protein-degrading bacteria growth.


Scientific Research Group of Radin Fidar Frda Co. 


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